Functionalization of a PC-TIR Biosensor for Label-Free cTnI Antigen Detection

dc.contributor.advisorYe, Jing Yong
dc.contributor.authorGonzalez, Christian
dc.contributor.committeeMemberGlickman, Randolph
dc.contributor.committeeMemberMiyahara, Yoichi
dc.contributor.committeeMemberHood, Robert L.
dc.creator.orcidhttps://orcid.org/0000-0001-8323-263X
dc.date.accessioned2024-02-09T21:57:21Z
dc.date.available2024-02-09T21:57:21Z
dc.date.issued2022
dc.descriptionThis item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstractDamaged myocardial cells release specific biomarkers, such as cardiac troponin I (cTnI) antigen, into the bloodstream. Sensitive detection of the cardiac biomarkers is a vital component for early detection of myocardial infarctions and drug-induced cardiotoxicity screenings. In this study, a compact standalone biosensing system was developed based on a Photonic-Crystal structure in a Total-Internal-Reflection (PC-TIR) configuration for label-free detection of cardiac biomarkers. To functionalize the PC-TIR sensor, (3-aminopropyl)triethoxysilane (APTES), carboxymethyl-dextran (CM-dextran), and 3-(triethoxysilyl)propylsuccinic anhydride (TESPSA) were immobilized on the sensing surface. These biochemical pathways were then tested to investigate and compare their binding affinity towards cTnI antibody and, ultimately, their ability to detect cTnI antigen. Additionally, cTnI antigen detection was investigated utilizing UV immobilization of Amino- and PolyT-modified aptamers on an APTES, (3-mercaptopropyl)trimethoxysilane (MPTMS) modified, and unmodified sensor surfaces. The cTnI antibody, Amino-modified aptamer, and Poly-T-modified aptamer running buffer's ionic strength and pH were then optimized to improve the binding affinity between antibodies/aptamers and their respective functionalized surfaces. After proper functionalization with either cTnI antibody or Amino- and Poly-T modified aptamers, the bioassays were evaluated for their respected capabilities to detect for cTnI antigen.
dc.description.departmentBiomedical Engineering
dc.format.extent75 pages
dc.format.mimetypeapplication/pdf
dc.identifier.isbn9798841761051
dc.identifier.urihttps://hdl.handle.net/20.500.12588/3780
dc.languageen
dc.subjectaptamer
dc.subjectcTnI antigen
dc.subjectfunctionalization
dc.subjectPC-TIR
dc.subjectTESPSA
dc.subjectUV
dc.subject.classificationBiomedical engineering
dc.subject.classificationBioengineering
dc.subject.classificationBiochemistry
dc.titleFunctionalization of a PC-TIR Biosensor for Label-Free cTnI Antigen Detection
dc.typeThesis
dc.type.dcmiText
dcterms.accessRightspq_closed
thesis.degree.departmentBiomedical Engineering
thesis.degree.grantorUniversity of Texas at San Antonio
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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