Development of an amniotic membrane tissue allograft for use as a wound dressing

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dc.contributor.advisorChristy, Robert J.
dc.contributor.advisorOng, Joo
dc.contributor.authorWehmeyer, Jennifer L.
dc.contributor.committeeMemberAgrawal, Mauli
dc.contributor.committeeMemberDean, David
dc.contributor.committeeMemberWang, Howard
dc.date.accessioned2024-03-08T17:36:05Z
dc.date.available2024-03-08T17:36:05Z
dc.date.issued2012
dc.descriptionThis item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstractNumerous techniques have been reported for preparing and sterilizing amniotic membrane (AM) for use in clinical applications; however, these preparations either do not render completely sterile tissue or are detrimental to molecules unique to the tissue matrix, thus compromising beneficial wound-healing properties of the AM graft. The objective of this work was to produce a sterile human AM tissue graft utilizing a novel preparation technique involving supercritical carbon dioxide (SCCO<sub>2</sub>). AM tissue was subjected to various sterilization treatments that optimized the duration of exposure to SCCO<sub>2</sub> and amount of peracetic acid (PAA) to achieve a sterility assurance level of 10<super>-6</super> log reduction in bacterial load. The effect of sterilization on the histological, biophysical, and biochemical properties of the AM graft was evaluated and compared to that of the native tissue. AMs treated with SCCO<sub>2</sub> were found to be excellent substrates for adipose-derived stem cell (ASC) attachment and proliferation in vitro. Human ASCs attached to all AM grafts after 24 hours of culture, irrespective of sterilization treatment, and continued to proliferate over the next few days. Furthermore, we evaluated the sterilized AM grafts with regards to integration into a full-thickness excision wound and their ability to promote wound healing in vivo. The current study's results indicate SCCO<sub>2</sub> can be used to sterilize AM tissue grafts while simultaneously preserving the biological attributes which make it appealing for use in numerous clinical and tissue engineering applications.
dc.description.departmentBiomedical Engineering
dc.format.extent103 pages
dc.format.mimetypeapplication/pdf
dc.identifier.isbn9781267843531
dc.identifier.urihttps://hdl.handle.net/20.500.12588/6149
dc.languageen
dc.subjectadipose derived stem cells
dc.subjectamniotic membrane
dc.subjectsupercritical carbon dioxide
dc.subjectwound healing
dc.subject.classificationBiomedical engineering
dc.titleDevelopment of an amniotic membrane tissue allograft for use as a wound dressing
dc.typeThesis
dc.type.dcmiText
dcterms.accessRightspq_closed
thesis.degree.departmentBiomedical Engineering
thesis.degree.grantorUniversity of Texas at San Antonio
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

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