Genetic manipulation of Francisella tularensis

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dc.contributor.authorZogaj, Xhavit
dc.contributor.authorKlose, Karl E.
dc.date.accessioned2023-06-30T16:27:18Z
dc.date.available2023-06-30T16:27:18Z
dc.date.issued2011-01-05
dc.description.abstractFrancisella tularensis is a facultative intracellular pathogen that causes the disease tularemia. F. tularensis subsp. tularensis causes the most severe disease in humans and has been classified as a Category A select agent and potential bioweapon. There is currently no vaccine approved for human use, making genetic manipulation of this organism critical to unraveling the genetic basis of pathogenesis and developing countermeasures against tularemia. The development of genetic techniques applicable to F. tularensis have lagged behind those routinely used for other bacteria, primarily due to lack of research and the restricted nature of the biocontainment required for studying this pathogen. However, in recent years, genetic techniques, such as transposon mutagenesis and targeted gene disruption, have been developed, that have had a dramatic impact on our understanding of the genetic basis of F. tularensis virulence. In this review, we describe some of the methods developed for genetic manipulation of F. tularensis.en_US
dc.description.departmentMolecular Microbiology and Immunologyen_US
dc.description.sponsorshipNational Institutes of Healthen_US
dc.identifier.citationZogaj, X., & Klose, K. (2011). Genetic Manipulation of Francisella tularensis. Frontiers in Microbiology, 1. doi:10.3389/fmicb.2010.00142en_US
dc.identifier.issn1664-302X
dc.identifier.otherhttps://doi.org/10.3389/fmicb.2010.00142
dc.identifier.urihttps://hdl.handle.net/20.500.12588/1967
dc.language.isoen_USen_US
dc.publisherFrontiers Mediaen_US
dc.relation.ispartofseriesFrontiers in Microbiology;Volume 1
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subjectFrancisella tularensisen_US
dc.subjecttularemiaen_US
dc.subjecttransposonen_US
dc.subjectallelic exchangeen_US
dc.subjectplasmiden_US
dc.subjecttargetronen_US
dc.subjectselect agenten_US
dc.titleGenetic manipulation of Francisella tularensisen_US
dc.typeArticleen_US

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