Proteomics and functional analysis of outer membrane vesicle from Pseudomonas aeruginosa under SOS

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dc.contributor.advisorWei, Tao
dc.contributor.authorMaredia, Reshma
dc.contributor.committeeMemberChaudry, G. Jilani
dc.contributor.committeeMemberWormley, Floyd
dc.date.accessioned2024-02-12T15:40:00Z
dc.date.available2024-02-12T15:40:00Z
dc.date.issued2011
dc.descriptionThis item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstractThe bacterial SOS response and outer membrane vesicle (OMV) production are conserved among Gram-negative bacteria, both contributing to virulence and biofilm development. SOS may link to OMV generation. To investigate such a connection, we conducted functional and proteomic analyses of OMVs from Pseudomonas aeruginosa. Functionally, OMVs mediated macrophage cytotoxicity and contributed to biofilm formation. OMV production and the cytotoxicity increased when the OMV-producing cells were SOS-induced by ciprofloxacin treatment, but decreased when LexA repressed SOS. Proteomic analysis revealed that proteins whose genes are known to be expressed during SOS were found predominantly in OMV proteins (OMVps) produced by the SOS-induced cells. These OMVps thus is termed SOS-OMVps. Many cytotoxic and biofilm-related proteins were also found in SOS-OMVps while some in the SOS-independent OMVps. Therefore, SOS appears to play a role in the antibiotic-stimulated OMV production, which contributes to cytotoxicity and biofilm formation.
dc.description.departmentIntegrative Biology
dc.format.extent64 pages
dc.format.mimetypeapplication/pdf
dc.identifier.isbn9781124866116
dc.identifier.urihttps://hdl.handle.net/20.500.12588/4558
dc.languageen
dc.subjectmacrophage cytotoxicity
dc.subjectouter membrane vesicle (OMV)
dc.subjectProteomic analysis
dc.subjectPseudomonas aeruginosa
dc.subjectSOS response
dc.subjectVirulence
dc.subject.classificationMicrobiology
dc.subject.classificationBiology
dc.titleProteomics and functional analysis of outer membrane vesicle from Pseudomonas aeruginosa under SOS
dc.typeThesis
dc.type.dcmiText
dcterms.accessRightspq_closed
thesis.degree.departmentIntegrative Biology
thesis.degree.grantorUniversity of Texas at San Antonio
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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