Isolation, characterization, and differentiaton of baboon adipose-derived stem cells: A nonhuman primate model for human stem cell therapy and regeneration

dc.contributor.advisorNavara, Christopher S.
dc.contributor.authorWrice, Nicole L.
dc.contributor.committeeMemberMcCarrey, John R.
dc.contributor.committeeMemberChristy, Robert J.
dc.date.accessioned2024-03-08T17:34:03Z
dc.date.available2024-03-08T17:34:03Z
dc.date.issued2013
dc.descriptionThis item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstractTherapies from both somatic and pluripotent stem cells are rapidly approaching clinical application. To ensure that these therapies can be used safely, a large animal model is critically needed. In this study, we demonstrated that baboons possess a reservoir of adipose-derived mesenchymal stem cells (bASCs) that can be isolated and further differentiated into various clinically relevant cell lineages. Baboon adipose tissue obtained during necropsy was processed according to established methods to obtain a stromal vascular fraction followed by the selection and expansion of bASCs. These stem cells were analyzed using FACS analysis and immunocytochemistry to detect a panel of positive and negative selection markers commonly used for the characterization of mesenchymal stem cell (MSC) populations in humans. Gene expression analysis of stem cell specific transcripts showed that bASCs maintained their multipotency over serial passages (P1-P6). Additionally, cells were differentiated in conditions known to induce adipogenic, osteogenic, and vasculogenic lineages confirmed through expression levels of differentiation specific genes, histological assays, and cell morphology. These experiments validate the baboon as an ideal pre-clinical model system for optimizing the efficacy and safety of stem cell therapies derived from ASCs.
dc.description.departmentIntegrative Biology
dc.format.extent74 pages
dc.format.mimetypeapplication/pdf
dc.identifier.isbn9781303114908
dc.identifier.urihttps://hdl.handle.net/20.500.12588/6018
dc.languageen
dc.subjectadipose-derived stem cell
dc.subjectbaboon
dc.subjectdifferentiation
dc.subjectmesenchymal stem cell
dc.subjectnonhuman primate
dc.subjectstem cell
dc.subject.classificationBiology
dc.subject.classificationMolecular biology
dc.subject.classificationCellular biology
dc.titleIsolation, characterization, and differentiaton of baboon adipose-derived stem cells: A nonhuman primate model for human stem cell therapy and regeneration
dc.typeThesis
dc.type.dcmiText
dcterms.accessRightspq_closed
thesis.degree.departmentIntegrative Biology
thesis.degree.grantorUniversity of Texas at San Antonio
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Wrice_utsa_1283M_11038.pdf
Size:
5.86 MB
Format:
Adobe Portable Document Format