Evaluation of Coccidioides Antigens, Enolase and Carboxypeptidase Y, as Vaccine Candidates Against Coccidioidomycosis

dc.contributor.advisorHung, Chiung-Yu
dc.contributor.authorBarnes, Christina
dc.contributor.committeeMemberSeshu, Janakiram
dc.contributor.committeeMemberYu, Jieh J.
dc.date.accessioned2024-01-25T22:34:06Z
dc.date.available2024-01-25T22:34:06Z
dc.date.issued2023
dc.descriptionThis item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstractCoccidioidomycosis (CM) is a pulmonary mycosis caused by Coccidioides spp. that impacts the health of residents who live or travel in the southwest regions of the United States. Vaccination against CM is a promising prevention that offers long-term immunity. A CD4+ T helper (Th) cell-mediated response is essential for vaccine-induced protective immunity. A previous study identified enolase (Enl) and carboxypeptidase Y (Cpya) in the cell wall of spherules as seroreactive. The goal of this study is to characterize and evaluate potential of the proteins of being a vaccine candidate. rEnl and rCpya were produced in an E. coli expression system and purified to ≥ 95% purity via nickel affinity chromatography. Purified proteins were used for IgELISA, rEnl shows to be Coccidioides specific compared to rCpya. rEnl was selected for T-cell recall assays to reveal its immunogenicity. ioGenetics algorithm shows that Enl contains 3 potential immunogenic epitopes (22-23 mers). Vaccinated HLA-DR4 transgenic mice with rEnl loaded in glucan-chitin particles (GCPs) with GCP-MSA as a control. Harvested splenocytes were prepared for T-cell recall assays with full-length rEnl and epitope peptides at a concentration of 100 nM. Findings indicate that Enl is a Coccidioides specific immunogenic antigen capable of eliciting a Th-specific cell mediated and a humoral response making it a good candidate to be used to develop a vaccine against CM.
dc.description.departmentIntegrative Biology
dc.format.extent48 pages
dc.format.mimetypeapplication/pdf
dc.identifier.isbn9798379575946
dc.identifier.urihttps://hdl.handle.net/20.500.12588/2469
dc.languageen
dc.subjectCoccidioidomycosis
dc.subjectPulmonary mycosis
dc.subjectLong-term immunity
dc.subjectImmunogenic antigen
dc.subject.classificationMicrobiology
dc.subject.classificationImmunology
dc.subject.classificationCellular biology
dc.titleEvaluation of Coccidioides Antigens, Enolase and Carboxypeptidase Y, as Vaccine Candidates Against Coccidioidomycosis
dc.typeThesis
dc.type.dcmiText
dcterms.accessRightspq_closed
thesis.degree.departmentIntegrative Biology
thesis.degree.grantorUniversity of Texas at San Antonio
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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