Sertoli Cell-Derived GDNF is Necessary for Quantitively Normal Spermatogenesis in Adult Mice

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dc.contributor.advisorHermann, Brian P.
dc.contributor.authorDoraji-Naseri, Behnam
dc.contributor.committeeMemberMcCarrey, John
dc.contributor.committeeMemberPhelix, Clyde F.
dc.date.accessioned2024-02-09T20:48:22Z
dc.date.available2024-02-09T20:48:22Z
dc.date.issued2017
dc.descriptionThis item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstractIn mammalian testes, spermatogenesis continually produces numerous spermatozoa dependent on the activity of spermatogonial stem cells (SSCs). Balance between self-renewal and differentiation fate decision by SSCs is necessary for maintaining the normal spermatogenesis and male fertility presumably occurs in response to external signal emanating from the niche such as Glial cell-lined derived neurotrophic factor (GDNF) that promotes SSC survival and self-renewal. For many years, it was considered that this GDNF was only produced by Sertoli cells. This idea was challenged recently by experiment demonstrating that production of GDNF by peritubular myoid (PM) cells is also essential for normal spermatogenesis. Surprisingly, though the hypothesis that in vivo production of GDNF by Sertoli cells is required for normal spermatogenesis, had never been tested. Therefore, we sought to address this hypothesis through conditional deletion of Gdnf in Sertoli cells of adult testes using tamoxifen-inducible Cre/lox genetics. Adult mice bearing a Sox9-CreERT2 transgene, a Rosa26-LacZ Cre reporter and with the following genotypes, Gdnf +/+, Gdnf fl/+ or Gdnf fl/fl were treated with tamoxifen to induce Sertoli cell-specific Gdnf deletion. Breeding studies showed that the fecundity of Gdnf Sertoli cell cKO mice was significantly lower (1.84 +/-0.46/litter) than controls (wild-type and Gdnf Sertoli cell cHet; 4.22+/-0.55 /litter; p ≤ 0.0012). In addition, testes weight of Gdnf Sertoli cell cKO mice was significantly lower (77.1 +/-7.46 mg) than controls (104.7+/-7.96 mg; p ≤ 0.017). Cre reporter (LacZ) expression and genotyping studies confirmed Cre-recombination and presence of floxed deleted Gdnf allele in cKO and cHet mice, respectively.
dc.description.departmentIntegrative Biology
dc.format.extent53 pages
dc.format.mimetypeapplication/pdf
dc.identifier.isbn9781369776911
dc.identifier.urihttps://hdl.handle.net/20.500.12588/3260
dc.languageen
dc.subject.classificationBiology
dc.titleSertoli Cell-Derived GDNF is Necessary for Quantitively Normal Spermatogenesis in Adult Mice
dc.typeThesis
dc.type.dcmiText
dcterms.accessRightspq_closed
thesis.degree.departmentIntegrative Biology
thesis.degree.grantorUniversity of Texas at San Antonio
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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