Advancing sample preparation, separation, and detection methods in capillary electrophoresis for the analysis of biologically active compounds

Capillary electrophoresis (CE) is a versatile analytical technique with a range of separation modes and detection options. Despite many advantages, its limits of detection must be improved for this relatively young separation technique to gain widespread use. Additionally, although the flexibility of CE is a great advantage, the optimization of so many separation parameters can be time-consuming and labor-intensive. The objectives of the projects in this Dissertation are to improve sensitivity and selectivity in the analysis of biologically active and environmentally relevant compounds and to efficiently optimize CE separation parameters. This Dissertation includes a description of three distinct projects. The first project shows that multivariate chemometric techniques are ideal for efficient optimization of separation conditions in CE. The second project demonstrates the importance of rational design of enzyme-modified electrodes for sensitive biosensors. Finally, the third project demonstrates the advantages of a preconcentration and derivatization method that will improve the limits of detection of primary amines. These contributions are important to the long-term goal of developing novel strategies to detect biologically active compounds. The projects also have a significant impact in the field of analytical chemistry, as they contribute to the general advancement of CE as a standard analytical technique.