Advancing sample preparation, separation, and detection methods in capillary electrophoresis for the analysis of biologically active compounds

dc.contributor.advisorGarcia, Carlos D.
dc.contributor.authorFelhofer, Jessica Lee
dc.contributor.committeeMemberAyon, Arturo A.
dc.contributor.committeeMemberGorski, Waldemar
dc.contributor.committeeMemberMusie, Ghezai T.
dc.date.accessioned2024-02-09T21:11:57Z
dc.date.available2024-02-09T21:11:57Z
dc.date.issued2012
dc.descriptionThis item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstractCapillary electrophoresis (CE) is a versatile analytical technique with a range of separation modes and detection options. Despite many advantages, its limits of detection must be improved for this relatively young separation technique to gain widespread use. Additionally, although the flexibility of CE is a great advantage, the optimization of so many separation parameters can be time-consuming and labor-intensive. The objectives of the projects in this Dissertation are to improve sensitivity and selectivity in the analysis of biologically active and environmentally relevant compounds and to efficiently optimize CE separation parameters. This Dissertation includes a description of three distinct projects. The first project shows that multivariate chemometric techniques are ideal for efficient optimization of separation conditions in CE. The second project demonstrates the importance of rational design of enzyme-modified electrodes for sensitive biosensors. Finally, the third project demonstrates the advantages of a preconcentration and derivatization method that will improve the limits of detection of primary amines. These contributions are important to the long-term goal of developing novel strategies to detect biologically active compounds. The projects also have a significant impact in the field of analytical chemistry, as they contribute to the general advancement of CE as a standard analytical technique.
dc.description.departmentChemistry
dc.format.extent100 pages
dc.format.mimetypeapplication/pdf
dc.identifier.isbn9781267842749
dc.identifier.urihttps://hdl.handle.net/20.500.12588/3574
dc.languageen
dc.subjectadsorption
dc.subjectamine
dc.subjectcapillary electrophoresis
dc.subjectcatalase
dc.subjectmultivariate optimization
dc.subjectpreconcentration
dc.subject.classificationChemistry
dc.subject.classificationAnalytical chemistry
dc.titleAdvancing sample preparation, separation, and detection methods in capillary electrophoresis for the analysis of biologically active compounds
dc.typeThesis
dc.type.dcmiText
dcterms.accessRightspq_closed
thesis.degree.departmentChemistry
thesis.degree.grantorUniversity of Texas at San Antonio
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

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