Maize-Produced Ag2 as a Subunit Vaccine for Valley Fever

dc.contributor.authorHayden, Celine A.
dc.contributor.authorHung, Chiung-Yu
dc.contributor.authorZhang, Hao
dc.contributor.authorNegron, Austin
dc.contributor.authorEsquerra, Raymond
dc.contributor.authorOstroff, Gary
dc.contributor.authorAbraham, Ambily
dc.contributor.authorLopez, Alejandro Gabriel
dc.contributor.authorGonzales, Juliet Elizabeth
dc.contributor.authorHoward, John A.
dc.creator.orcidhttps://orcid.org/0000-0003-1091-3420en_US
dc.date.accessioned2023-06-29T16:30:27Z
dc.date.available2023-06-29T16:30:27Z
dc.date.issued2019-04-23
dc.description.abstractCoccidioides is the causative agent of San Joaquin Valley fever, a fungal disease prevalent in the semiarid regions of the Americas. Efforts to develop a fungal vaccine over the last 2 decades were unsuccessful. A candidate antigen, Antigen 2 (Ag2), is notoriously difficult to express in Escherichia coli, and this study sought to accumulate the antigen at high levels in maize. Transformed maize lines accumulated recombinant Ag2 at levels >1 g/kg. Mice immunized with this antigen and challenged with live Coccidioides arthroconidia showed a reduction in the fungal load when Ag2 derived from either E. coli or maize was loaded into glucan chitin particles. A fusion of Ag2 to dendritic cell carrier peptide (DCpep) induced a T-helper type 17 response in the spleen when orally delivered, indicative of a protective immune response. The maize production platform and the glucan chitin particle adjuvant system show promise for development of a Coccidioides vaccine, but further testing is needed to fully assess the optimal method of administration.en_US
dc.description.departmentMolecular Microbiology and Immunologyen_US
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases, National Institutes of Health; National Institutes of Health MARC program; Genentech Foundation Scholars Programen_US
dc.identifier.citationHayden, C. A., Hung, C.-Y., Zhang, H., Negron, A., Esquerra, R., Ostroff, G., . . . Howard, J. A. (2019). Maize-Produced Ag2 as a Subunit Vaccine for Valley Fever. The Journal of Infectious Diseases, 220(4), 615-623. doi:10.1093/infdis/jiz196en_US
dc.identifier.issn1537-6613
dc.identifier.otherhttps://doi.org/10.1093/infdis/jiz196
dc.identifier.urihttps://hdl.handle.net/20.500.12588/1963
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofseriesThe Journal of Infectious Diseases;Volume 220, Issue 4
dc.subjectvalley feveren_US
dc.subjectCoccidioidesen_US
dc.subjectsubunit vaccineen_US
dc.subjectmaize-produced vaccineen_US
dc.subjectplant vaccineen_US
dc.subjectglucan chitin particlesen_US
dc.subjectbioencapsulationen_US
dc.titleMaize-Produced Ag2 as a Subunit Vaccine for Valley Feveren_US
dc.typeArticleen_US

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