Bmp-2 - tethered polyelectrolyte coated scaffolds for bone tissue regeneration




Shiels, Stefanie M.

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The combination of therapeutic growth factor and tissue platform is a highly researched topic for replace of autogenic and allogenic bone grafts. Hydroxyapatite scaffolds combined with rhBMP-2 have to potential to regenerate functional bone tissue while reducing the therapeutic dose. The goal of this research project was to develop a rhBMP-2 coated implant for bone tissue regeneration. To investigate this objective, the coating chemistry, in vitro biological signaling propagation, and in vivo performance were evaluated. From these studies it was concluded that a polyelectrolyte coating on HAp can retain the rhBMP-2 to the surface of the HAp and encourage osteoblast attachment. The in vitro evaluation revealed that the rhBMP-2 bound to a polyelectrolyte coating on HAp caused a delayed osteogenic initiation, but increased the BMP cell receptor number and osteopontin production. This revealed that the signal propagation was activated by a signaling complex associated with Smad-dependent signaling pathway. Finally, after 8 weeks implanted into a rabbit femoral condyle, it was concluded that the rhBMP-2 coating didn't increase the bone volume, but did increase vascular recruitment around the implant. From these findings, it was concluded that a rhBMP-2 coated implant is a viable step toward the regeneration of functional bone tissue using synthetic means.


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BMP-2, Hydroxyapatite, Tissue Engineering



Biomedical Engineering