The Role of IL-17A as a Potential Mediator of Chlamydial Pathogenesis

dc.contributor.advisorArulanandam, Bernard P.
dc.contributor.authorArkatkar, Tanvi
dc.contributor.committeeMemberGuentzel, Neal
dc.contributor.committeeMemberChambers, James
dc.descriptionThis item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstractPURPOSE: IL-17 has been reported to play an important role in host defenses against pulmonary chlamydial infections by promoting a Th1 response. However, molecular mechanisms associated with IL-17 in genital chlamydial infection have not been well characterized and were the purpose of this study. METHODS: C57BL/6 wild type (WT) and IL-17-/- mice were intravaginally challenged with C. muridarum (CM) and differences in infiltrating lymphocytes, T and B cell responses and miRNA expression were analyzed. We isolated RNA from the lower genital tracts of CM infected WT and IL-17A-/- mice and analyzed the gene transcripts with a high throughput qPCR based array for 84 genes associated with T and B cell activation. RESULTS: Pathology was decreased at day 80 post challenge in IL-17A-/- compared to WT mice. Six genes were significantly (p<0.05) regulated at day 6 post-challenge including ICAM1 and IL-1&#946; which are known mediators of pathology. These genes were further analyzed (miRNA based in silico prediction software); miRNA 214 was significantly upregulated in IL-17-/- mice, which has two target binding sites for the downregulated ICAM-1 gene. Additionally, we derived miR 214 over expressing genital cells which decreased ICAM-1 with CM infection in vitro. Furthermore, recruitment of neutrophils in IL-17 A-/- was reduced compared to WT mice. CONCLUSION: Decreased pathology in IL-17 A-/- mice may be attributed to reduced expression of ICAM-1 leading to reduction in neutrophil infiltration at the early stage of genital infection.
dc.description.departmentIntegrative Biology
dc.format.extent34 pages
dc.titleThe Role of IL-17A as a Potential Mediator of Chlamydial Pathogenesis
dcterms.accessRightspq_closed Biology of Texas at San Antonio of Science


Original bundle

Now showing 1 - 1 of 1
No Thumbnail Available
612.91 KB
Adobe Portable Document Format