Role of cdGMP in Francisella novicida biofilm formation

dc.contributor.advisorKlose, Karl E.
dc.contributor.authorWyatt, Geoffrey
dc.contributor.committeeMemberSeshu, Janakiram
dc.contributor.committeeMemberGuentzel, Neal
dc.date.accessioned2024-03-08T17:34:05Z
dc.date.available2024-03-08T17:34:05Z
dc.date.issued2012
dc.descriptionThis item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstract<italic>Francisella tularensis</italic> is a gram-negative intracellular pathogen that is highly virulent in humans causing the disease tularemia. <italic>F. novicida</italic> is closely-related to <italic>F. tularensis</italic> but is avirulent in humans. Within a cluster of six genes that is specific to <italic>F. novicida</italic> are two genes, <italic>cdgA</italic> and <italic>cdgB</italic>, that encode proteins with diguanylate cyclase (DGC) and phosphodiesterase (PDE) domains. The <italic>F. tularensis</italic> genome does not encode any DGC or PDE containing proteins. DGCs and PDEs modulate the synthesis and degradation of cyclic di-GMP (cdGMP). cdGMP is a signaling molecule involved in the shift between planktonic and biofilm growth phases. An <italic>F. novicida</italic> mutant strain lacking both <italic>cdgA</italic> and <italic>cdgB</italic> is defective for biofilm formation. Although they contain both CDG and PDE domains, biofilm studies suggest that CdgA acts primarily as a PDE, while CdgB acts primarily as a DGC. Expressing CdgB from a plasmid stimulates <italic>F. novicida</italic> biofilm formation, even in a strain lacking the biofilm regulator <italic>qseB</italic>. qRT-PCR analysis confirmed that QseB regulates <italic>cdgA</italic> and <italic>cdgB</italic> transcription, suggesting a signaling cascade controlling cdGMP levels. We suggest that differences in cdGMP signaling may contribute to the relative virulence of <italic>Francisella</italic> species.
dc.description.departmentIntegrative Biology
dc.format.extent32 pages
dc.format.mimetypeapplication/pdf
dc.identifier.isbn9781267843555
dc.identifier.urihttps://hdl.handle.net/20.500.12588/6024
dc.languageen
dc.subject.classificationMicrobiology
dc.titleRole of cdGMP in Francisella novicida biofilm formation
dc.typeThesis
dc.type.dcmiText
dcterms.accessRightspq_closed
thesis.degree.departmentIntegrative Biology
thesis.degree.grantorUniversity of Texas at San Antonio
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Wyatt_utsa_1283M_10964.pdf
Size:
527.92 KB
Format:
Adobe Portable Document Format