Construction of targeted insertion mutations in Francisella tularensis subsp. novicida

dc.contributor.authorLiu, Jirong
dc.contributor.authorZogaj, Xhavit
dc.contributor.authorBarker, Jeffrey R.
dc.contributor.authorKlose, Karl E.
dc.date.accessioned2023-06-15T19:01:30Z
dc.date.available2023-06-15T19:01:30Z
dc.date.issued2007-10
dc.description.abstractFrancisella tularensis is one of the most deadly bacterial agents, yet most of the genetic determinants of pathogenesis are still unknown. We have developed an efficient targeted mutagenesis strategy in the model organism F. tularensis subsp. novicida by utilizing universal priming of optimized antibiotic resistance cassettes and splicing by overlap extension (SOE). This process enables fast and efficient construction of targeted insertion mutations in F. tularensis subsp. novicida that have characteristics of nonpolar mutations; optimized targeted mutagenesis strategies will promote the study of this mysterious bacterium and facilitate vaccine development against tularemia. Moreover, the general strategy of gene disruption by PCR-based antibiotic resistance cassette insertion is broadly applicable to many bacterial species.en_US
dc.description.departmentMolecular Microbiology and Immunology
dc.description.departmentNeuroscience, Developmental and Regenerative Biology
dc.description.sponsorshipNational Institute of Allergies and Infectious Diseases; National Institutes of Health; Department of Health and Human Servicesen_US
dc.identifier.citationLiu, J., Zogaj, X., Barker, J. R., & Klose, K. E. (2007). Construction of targeted insertion mutations in Francisella tularensis subsp. novicida. BioTechniques, 43(4), 487-492. doi:10.2144/000112574en_US
dc.identifier.issn1940-9818
dc.identifier.otherhttps://doi.org/10.2144/000112574
dc.identifier.urihttps://hdl.handle.net/20.500.12588/1889
dc.language.isoen_USen_US
dc.publisherFuture Science Groupen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.titleConstruction of targeted insertion mutations in Francisella tularensis subsp. novicidaen_US
dc.typeArticleen_US

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