Microsatellite genotyping of clinical Candida parapsilosis isolates

Date
2017-12
Authors
Badali, Hamid
Rezaie, Sassan
Meis, Jacques F.
Agha Kuchak Afshari, Setareh
Modiri, Mona
Hagen, Ferry
Moazeni, Maryam
Mohammadi, Rasoul
Khodavaisy, Sadegh
Journal Title
Journal ISSN
Volume Title
Publisher
Mazandaran University of Medical Sciences
Abstract

Background and Purpose: Candida parapsilosis is a predominant species found in nosocomial infection, particularly in hospitalized patients. The molecular epidemiology of the clinical strains of this species has not been well studied. The present study was performed with the aim of investigating the microsatellite genotyping of Candida parapsilosis among the Iranian clinical isolates.

Materials and Methods: This study was conducted on 81 independent clinical C. parapsilosis isolates that were genotyped by using a panel of six microsatellite markers.

Results: The short tandem repeat (STR) typing of clinical C. parapsilosis isolates demonstrated 68 separate genotypes, among which 57 genotypes were observed once and the remaining 11 cases were identified for multiple times. The Simpson’s diversity index for the panel of combined six markers yielded a diversity index of 0.9951. The heterogeneity was observed among the Iranian and the Netherlands clinical C. parapsilosis isolates.

Conclusion: As the findings indicated, the clinical C. parapsilosis isolates from Iran showed a high genetic diversity. It can be concluded that molecular epidemiology could be useful for screening during outbreak investigation where C. parapsilosis is involved.

Description
Keywords
Candida parapsilosis, genotyping, Iran, microsatellite
Citation
Badali H, Rezaie S, Meis JF, Agha Kuchak Afshari S, Modiri M, Hagen F, Moazeni M, Mohammadi R, Khodavaisy S. Microsatellite genotyping of clinical Candida parapsilosis isolates. Curr Med Mycol. 2017 Dec;3(4):15-20. doi: 10.29252/cmm.3.4.15. PMID: 29707674; PMCID: PMC5917096.
Department
Molecular Microbiology and Immunology