Generation of SIV-resistant CD4+ t cells modified with CCR5 specific Zinc-Finger Nucleases in the rhesus macaque

dc.contributor.advisorHeidner, Hans
dc.contributor.authorLentz, Peter
dc.contributor.committeeMemberLopez-Ribot, Jose
dc.contributor.committeeMemberGauduin, Marie-Claire
dc.date.accessioned2024-02-02T21:37:58Z
dc.date.available2024-02-02T21:37:58Z
dc.date.issued2012
dc.descriptionThe author has granted permission for their work to be available to the general public.
dc.description.abstractIn this project, we wanted to see if it was possible to engineer cells that could mimic some of what was seen in the Berlin Patient. The Berlin Patient was an HIV positive man diagnosed with AML that needed bone marrow transplants to live. His doctor decided it could be beneficial to see if there was a bone marrow donor that was possibly CCR5 negative and transplant that bone marrow into the patient. They were successful in finding a donor and after three years, the patient is found to have no detectable levels of HIV in his blood stream or other reservoirs where it is usually found in an infected individual, this is even after removing HAART treatments. Our goal was to use a style of gene editing using Zinc-Finger Nucleases (ZFN) that could delete the CCR5 region of the gene to have cells that lacked the necessary receptor for SIV envelope fusion. We chose to use CD4+ T cells since these are the targets of HIV and SIV as well as using CD34+ cells isolated from rhesus macaque bone marrow. The long-term goal of this idea is to have engineered SIV resistant cells that could possibly be transplanted back into the monkey and see if SIV resistance was possible.
dc.description.departmentIntegrative Biology
dc.format.extent71 pages
dc.format.mimetypeapplication/pdf
dc.identifier.isbn9781267343871
dc.identifier.urihttps://hdl.handle.net/20.500.12588/2819
dc.languageen
dc.subjectBerlin Patient
dc.subjectBone marrow
dc.subjectCCR5
dc.subjectRhesus macaque
dc.subjectSIV
dc.subjectZinc-finger nuclease
dc.subject.classificationBiology
dc.subject.classificationGenetics
dc.subject.classificationImmunology
dc.titleGeneration of SIV-resistant CD4+ t cells modified with CCR5 specific Zinc-Finger Nucleases in the rhesus macaque
dc.typeThesis
dc.type.dcmiText
dcterms.accessRightspq_OA
thesis.degree.departmentIntegrative Biology
thesis.degree.grantorUniversity of Texas at San Antonio
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Lentz_utsa_1283M_10801.pdf
Size:
1.42 MB
Format:
Adobe Portable Document Format