A bacterial two-hybrid system that utilizes Gateway cloning for rapid screening of protein-protein interactions

dc.contributor.authorKarna, S. L. Rajasekhar
dc.contributor.authorZogaj, Xhavit
dc.contributor.authorBarker, Jeffrey R.
dc.contributor.authorSeshu, Janakiram
dc.contributor.authorDove, Simon L.
dc.contributor.authorKlose, Karl E.
dc.date.accessioned2023-06-15T19:22:22Z
dc.date.available2023-06-15T19:22:22Z
dc.date.issued2010-11
dc.description.abstractComprehensive clone sets representing the entire genome now exist for a large number of organisms. The Gateway entry clone sets are a particularly useful means to study gene function, given the ease of introduction into any Gateway-suitable destination vector. We have adapted a bacterial two-hybrid system for use with Gateway entry clone sets, such that potential interactions between proteins encoded within these clone sets can be determined by new destination vectors. We show that utilizing the Gateway clone sets for Francisella tularensis and Vibrio cholerae, known interactions between F. tularensis IglA and IglB and V. cholerae VipA and VipB could be confirmed with these destination vectors. Moreover, the introduction of unique tags into each vector allowed for visualization of the expressed hybrid proteins via Western immunoblot. This Gateway-suitable bacterial two-hybrid system provides a new tool for rapid screening of protein-protein interactions.en_US
dc.description.departmentMolecular Microbiology and Immunologyen_US
dc.description.departmentNeuroscience, Developmental and Regenerative Biology
dc.description.sponsorshipNational Institutes of Healthen_US
dc.identifier.citationKarna, S. L. R., Zogaj, X., Barker, J. R., Seshu, J., Dove, S. L., & Klose, K. E. (2010). A bacterial two-hybrid system that utilizes Gateway cloning for rapid screening of protein-protein interactions. BioTechniques, 49(5), 831-833. doi:10.2144/000113539en_US
dc.identifier.issn1940-9818
dc.identifier.otherhttps://doi.org/10.2144/000113539
dc.identifier.urihttps://hdl.handle.net/20.500.12588/1890
dc.language.isoen_USen_US
dc.publisherFuture Science Groupen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.titleA bacterial two-hybrid system that utilizes Gateway cloning for rapid screening of protein-protein interactionsen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Karna 2010 - A bacterial two-hybrid system that utilizes Gateway cloning for rapid screening of protein-protein interactions.pdf
Size:
1.31 MB
Format:
Adobe Portable Document Format
Description:

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.86 KB
Format:
Item-specific license agreed upon to submission
Description: