Multivalent Recombinant Protein Vaccine against Coccidioidomycosis

dc.contributor.authorTarcha, Eric J.
dc.contributor.authorBasrur, Venkatesha
dc.contributor.authorHung, Chiung-Yu
dc.contributor.authorGardner, Malcolm J.
dc.contributor.authorCole, Garry T.
dc.creator.orcidhttps://orcid.org/0000-0003-1091-3420en_US
dc.date.accessioned2023-06-28T15:13:45Z
dc.date.available2023-06-28T15:13:45Z
dc.date.issued2006-10-01
dc.description.abstractCoccidioidomycosis is a human respiratory disease that is endemic to the southwestern United States and is caused by inhalation of the spores of a desert soilborne fungus. Efforts to develop a vaccine against this disease have focused on identification of T-cell-reactive antigens derived from the parasitic cell wall which can stimulate protective immunity against Coccidioides posadasii infection in mice. We previously described a productive immunoproteomic/bioinformatic approach to the discovery of vaccine candidates which makes use of the translated genome of C. posadasii and a computer-based method of scanning deduced sequences of seroreactive proteins for epitopes that are predicted to bind to human major histocompatibility (MHC) class II-restricted molecules. In this study we identified a set of putative cell wall proteins predicted to contain multiple, promiscuous MHC II binding epitopes. Three of these were expressed by Escherichia coli, combined in a vaccine, and tested for protective efficacy in C57BL/6 mice. Approximately 90% of the mice survived beyond 90 days after intranasal challenge, and the majority cleared the pathogen. We suggest that the multicomponent vaccine stimulates a broader range of T-cell clones than the single recombinant protein vaccines and thereby may be capable of inducing protection in an immunologically heterogeneous human population.en_US
dc.description.departmentMolecular Microbiology and Immunologyen_US
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases, National Institutes of Health; Margaret Batts Tobin Foundation of San Antonio, Texasen_US
dc.identifier.citationTarcha, E. J., Basrur, V., Hung, C.-Y., Gardner, M. J., & Cole, G. T. (2006). Multivalent Recombinant Protein Vaccine against Coccidioidomycosis. Infection and Immunity, 74(10), 5802-5813. doi:10.1128/iai.00961-06en_US
dc.identifier.issn1098-5522
dc.identifier.otherhttps://doi.org/10.1128/iai.00961-06
dc.identifier.urihttps://hdl.handle.net/20.500.12588/1951
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofseriesInfection and Immunity;Vol. 74, No. 10
dc.subjectCoccidioidomycosisen_US
dc.subjectCoccidioides posadasiien_US
dc.subjectCoccidioidesen_US
dc.subjectvaccineen_US
dc.titleMultivalent Recombinant Protein Vaccine against Coccidioidomycosisen_US
dc.typeArticleen_US

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