Use of Amplified Fragment Length Polymorphism To Identify 42 Cladophialophora Strains Related to Cerebral Phaeohyphomycosis with In Vitro Antifungal Susceptibility

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dc.contributor.authorBadali, Hamid
dc.contributor.authorde Hoog, G. Sybren
dc.contributor.authorCurfs-Breuker, Ilse
dc.contributor.authorKlaassen, Corné H. W.
dc.contributor.authorMeis, Jacques F.
dc.creator.orcidhttps://orcid.org/0000-0002-6010-8414en_US
dc.date.accessioned2023-07-17T18:19:14Z
dc.date.available2023-07-17T18:19:14Z
dc.date.issued2010-07-01
dc.description.abstractThe amplified fragment length polymorphism technique has been applied to identify neurotropic chaetothyrialean black yeasts and relatives from clinical sources. Cladophialophora bantiana, C. emmonsii, C. arxii, C. devriesii, and C. modesta, previously identified on the basis of sequencing and phenotypic and physiological criteria, were confirmed by cluster analysis, demonstrating the clear separation of C. bantiana as a rather homogeneous group from the other species. C. bantiana is a neurotropic fungus causing cerebral abscesses with a mortality of up to 70%. Successful therapy consists of neurosurgical intervention and optimal antifungal therapy. Since the latter is not clearly defined in a large series, we tested the in vitro activities of eight antifungal drugs against clinical isolates of C. bantiana (n = 37), C. modesta (n = 2), C. arxii (n = 1), C. emmonsii (n = 1), and C. devriesii (n = 1), all of which had caused invasive infections. The resulting MIC90s for all neurotropic C. bantiana strains were as follows, in increasing order: posaconazole, 0.125 μg/ml; itraconazole, 0.125 μg/ml; isavuconazole, 0.5 μg/ml; amphotericin B, 1 μg/ml; voriconazole, 2 μg/ml; anidulafungin, 2 μg/ml; caspofungin, 4 μg/ml; and fluconazole, 64 μg/ml. On the basis of these in vitro results and the findings of previous clinical and animal studies, posaconazole seems to be a good alternative to the standard treatment, amphotericin B, for C. bantiana cerebral infections. The new agent isavuconazole, which is also available as an intravenous preparation, has adequate activity against C. bantiana.en_US
dc.description.departmentMolecular Microbiology and Immunologyen_US
dc.description.sponsorshipMinistry of Health and Medical Education of the Islamic Republic of Iran; School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; Basilea Pharmaceuticals, Basel, Switzerlanden_US
dc.identifier.citationBadali, H., de Hoog, G. S., Curfs-Breuker, I., Klaassen, C. H. W., & Meis, J. F. (2010). Use of Amplified Fragment Length Polymorphism To Identify 42 Cladophialophora Strains Related to Cerebral Phaeohyphomycosis with In Vitro Antifungal Susceptibility. Journal of Clinical Microbiology, 48(7), 2350-2356. doi:10.1128/jcm.00653-10en_US
dc.identifier.issn1098-660X
dc.identifier.otherhttps://doi.org/10.1128/JCM.00653-10
dc.identifier.urihttps://hdl.handle.net/20.500.12588/1994
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofseriesJournal of Clinical Microbiology;Vol. 48, No. 7
dc.subjectCladophialophoraen_US
dc.subjectcerebral phaeohyphomycosisen_US
dc.subjectantifungal susceptibilityen_US
dc.titleUse of Amplified Fragment Length Polymorphism To Identify 42 Cladophialophora Strains Related to Cerebral Phaeohyphomycosis with In Vitro Antifungal Susceptibilityen_US
dc.typeArticleen_US

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