Viscosity Performance Testing of a Redesigned, Portable, Nonelectronic Versatile Oxygenating Perfusion System
This thesis provides an overview on the redesign and characterization of a versatile oxygenating prefusion system (VOPS) that is frugal, portable, and nonelectronic. This VOPS can provide oxygenated perfusion to a range of biological tissues that have been isolated from a donor for transplantation. The device is unique as it combines the oxygenator and pump into a single component, reducing the costs and complexity typically associated with perfusion devices. The need for this device is motivated by the number of patients on the organ transplant waitlist who do not receive a life-saving transplant. Each year, thousands of organs are harvested for transplantation but fail to be transplanted due to inadequate preservation techniques available. Static cold storage, the clinical gold standard, is the current method to preserve an organ to be transplanted by bathing an organ in a preservation solution and cooling it to a hypothermic state to reduce metabolic function. Machine perfusion, an alternative organ preservation technique that involves introducing flow and physiological pressures into the organ vasculature being preserved, has shown better organ preservation outcomes over static cold storage. Our VOPS device needs to compatibly function with several of the preservation solutions that are currently used in organ preservation, motivating these studies wherein the device performance was measured across a range of solutions that mimic the viscosity of these existing preservation solutions. The device perfusion pressures, oxygenation, and flow rates were evaluated with a PVC element that mimicked the vascular resistance of a human kidney. The device was successfully able to reach the perfusion parameters that have been established by other groups to clinically preserve kidneys at all different viscosities tested. This success provides strong evidence on the versatile functionality of the VOPS.