Study of virulence factors associated with Acinetobacter baumannii systemic and gastrointestinal infections

dc.contributor.advisorArulanandam, Bernard P.
dc.contributor.authorKetter, Patrick Morgan
dc.contributor.committeeMemberCap, Andrew
dc.contributor.committeeMemberChambers, James
dc.contributor.committeeMemberEppinger, Mark
dc.contributor.committeeMemberSeshu, Janakiram
dc.contributor.committeeMemberGuentzel, M. Neal
dc.date.accessioned2024-02-12T14:40:51Z
dc.date.available2024-02-12T14:40:51Z
dc.date.issued2015
dc.descriptionThis item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstractMulti-drug resistant Acinetobacter baumannii is among the most prevalent bacterial pathogens associated with trauma-related wound and bloodstream infections. Gastrointestinal (GI) tract colonization may contribute to emergence of these MDR A. baumannii isolates. Furthermore, while neutrophils are known to be essential for A. baumannii clearance, little else is known about either the protective host immune response to this pathogen, or virulence factors associated with it. In these studies, we set out to determine the host response to virulence factors associated with A. baumannii with the following specific aims: 1) elucidate the role of pentraxin 3 (PTX3) found in neutrophilic granules in A. baumannii sepsis, 2) elucidate the role of SIgA in A. baumannii GI tract colonization, and 3) characterize the interaction between A. baumannii and SIgA. First, utilizing an intraperitoneal sepsis challenge model, we observed that elevated PTX3 production following A. baumannii challenge was associated with increased morbidity and mortality. Next, we observed enhancement of A. baumannii GI tract colonization in the presence of SIgA using an oral GI challenge model. Finally, using RNAseq and in vitro culture, we found that thioredoxin may contribute to GI tract colonization. These studies ultimately lead us to generate a thioredoxin deletion mutant and corresponding complemented strain for use in future studies to further elucidate the role of thioredoxin in A. bauamannii GI tract colonization.
dc.description.departmentIntegrative Biology
dc.format.extent146 pages
dc.format.mimetypeapplication/pdf
dc.identifier.isbn9781339034485
dc.identifier.urihttps://hdl.handle.net/20.500.12588/4055
dc.languageen
dc.subjectAcinetobacter baumannii
dc.subjectcoagulopathy
dc.subjectgastrointestinal
dc.subjectPTX3
dc.subjectSIgA
dc.subjectthioredoxin
dc.subject.classificationImmunology
dc.subject.classificationMicrobiology
dc.subject.classificationMolecular biology
dc.subject.lcshAcinetobacter infections
dc.subject.lcshGastrointestinal system -- Diseases
dc.titleStudy of virulence factors associated with Acinetobacter baumannii systemic and gastrointestinal infections
dc.typeThesis
dc.type.dcmiText
dcterms.accessRightspq_closed
thesis.degree.departmentIntegrative Biology
thesis.degree.grantorUniversity of Texas at San Antonio
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

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