Transcription Regulation of Candida albicans Examining Filamentation Following Hyphae Induction

Date

2020

Authors

Keith, Dwayne

Journal Title

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Abstract

Candida albicans is a fungal pathogen that is part of the human microbiota. It is an opportunistic organism that grows on mucosal surfaces of the gastrointestinal and genitourinary tracts. A few of the common diseases caused by C. albicans are thrush, yeast infections, multiple types of candidiasis, and is also known to cause life threatening infections in the immunocompromised. Hyphae morphogenesis, changing from yeast to hyphae, is key to its virulence. The ability to form hyphae allows C. albicans to form biofilms, evade phagocytosis by the immune system, and penetrate tissue eventually leading to systemic infections. UME6 is a transcriptional factor that plays a direct role in hyphal elongation and germ tube formation. In a previous study, it was found that BRG1 could not filament on YPD when inserted into a ume6Δ mutant, these results were used to determined that UME6 was downstream of BRG1. We decided to investigate this relationship further and inserted the UME6 gene into a plasmid that had a tet-operator and trans-activator to regulate expression, then introduced the tet-UME6 allele into a brg1Δ strain and the previously engineered tet-BRG1 allele into a ume6Δ strain. We then subjected the brgΔ-tet-UME6 and ume6Δ-tet-BRG1 strains to multiple hyphae inducing conditions. We than determined that UME6 and BRG1 are both required by the other to filament except under one hyphae inducing condition. From these experiments we concluded that both genes form a feedback loop, although more research is required.

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Keywords

Transcription regulation, Candida albicans, Filamentation, Hyphae induction

Citation

Department

Integrative Biology