Pimentel-Acosta, Citlalic A.Ramírez-Salcedo, JorgeMorales-Serna, Francisco NeptalíFajer-Ávila, Emma J.Chávez-Sánchez, CristinaLara, Humberto H.García-Gasca, Alejandra2021-04-192021-04-192020-08-16International Journal of Molecular Sciences 21 (16): 5889 (2020)https://hdl.handle.net/20.500.12588/512The mechanisms of action of silver nanoparticles (AgNPs) in monogenean parasites of the genus Cichlidogyrus were investigated through a microarray hybridization approach using genomic information from the nematode Caenorhabditis elegans. The effects of two concentrations of AgNPs were explored, low (6 µg/L Ag) and high (36 µg/L Ag). Microarray analysis revealed that both concentrations of AgNPs activated similar biological processes, although by different mechanisms. Expression profiles included genes involved in detoxification, neurotoxicity, modulation of cell signaling, reproduction, embryonic development, and tegument organization as the main biological processes dysregulated by AgNPs. Two important processes (DNA damage and cell death) were mostly activated in parasites exposed to the lower concentration of AgNPs. To our knowledge, this is the first study providing information on the sub-cellular and molecular effects of exposure to AgNPs in metazoan parasites of fish.Attribution 4.0 United Stateshttps://creativecommons.org/licenses/by/4.0/silver nanoparticlesmonogenean parasitesgene expressionmicroarray analysisCaenorhabditis elegansCichlidogyrusArticle2021-04-19