Nanocurcumin-Loaded UCNPs for Cancer Theranostics: Physicochemical Properties, In Vitro Toxicity, and In Vivo Imaging Studies
dc.contributor.author | Lakshmanan, Anbharasi | |
dc.contributor.author | Akasov, Roman A. | |
dc.contributor.author | Sholina, Natalya V. | |
dc.contributor.author | Demina, Polina A. | |
dc.contributor.author | Generalova, Alla N. | |
dc.contributor.author | Gangadharan, Ajithkumar | |
dc.contributor.author | Sardar, Dhiraj K. | |
dc.contributor.author | Lankamsetty, Krishna Bharat | |
dc.contributor.author | Khochenkov, Dmitry A. | |
dc.contributor.author | Khaydukov, Evgeny V. | |
dc.contributor.author | Gudkov, Sergey V. | |
dc.contributor.author | Jayaraman, Manonmani | |
dc.contributor.author | Jayaraman, Senthilselvan | |
dc.date.accessioned | 2021-09-25T23:33:43Z | |
dc.date.available | 2021-09-25T23:33:43Z | |
dc.date.issued | 2021-08-29 | |
dc.date.updated | 2021-09-25T23:33:44Z | |
dc.description.abstract | Formulation of promising anticancer herbal drug curcumin as a nanoscale-sized curcumin (nanocurcumin) improved its delivery to cells and organisms both in vitro and in vivo. We report on coupling nanocurcumin with upconversion nanoparticles (UCNPs) using Poly (lactic-co-glycolic Acid) (PLGA) to endow visualisation in the near-infrared transparency window. Nanocurcumin was prepared by solvent-antisolvent method. NaYF4:Yb,Er (UCNP1) and NaYF4 :Yb,Tm (UCNP2) nanoparticles were synthesised by reverse microemulsion method and then functionalized it with PLGA to form UCNP-PLGA nanocarrier followed up by loading with the solvent-antisolvent process synthesized herbal nanocurcumin. The UCNP samples were extensively characterised with XRD, Raman, FTIR, DSC, TGA, UV-VIS-NIR spectrophotometer, Upconversion spectrofluorometer, HRSEM, EDAX and Zeta Potential analyses. UCNP1-PLGA-nanocurcumin exhibited emission at 520, 540, 660 nm and UCNP2-PLGA-nanocurmin showed emission at 480 and 800 nm spectral bands. UCNPPLGA-nanocurcumin incubated with rat glioblastoma cells demonstrated moderate cytotoxicity, 60–80% cell viability at 0.12–0.02 mg/mL marginally suitable for therapeutic applications. The cytotoxicity of UCNPs evaluated in tumour spheroids models confirmed UCNP-PLGA-nanocurcumin therapeutic potential. As-synthesised curcumin-loaded nanocomplexes were administered in tumourbearing laboratory animals (Lewis lung cancer model) and showed adequate contrast to enable in vivo and ex vivo study of UCNP-PLGA-nanocurcumin bio distribution in organs, with dominant distribution in the liver and lungs. Our studies demonstrate promise of nanocurcumin-loaded upconversion nanoparticles for theranostics applications. | |
dc.description.department | Physics and Astronomy | |
dc.identifier | doi: 10.3390/nano11092234 | |
dc.identifier.citation | Nanomaterials 11 (9): 2234 (2021) | |
dc.identifier.uri | https://hdl.handle.net/20.500.12588/686 | |
dc.rights | Attribution 4.0 United States | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | upconversion nanoparticles | |
dc.subject | theranostics | |
dc.subject | nanocurcumin | |
dc.subject | herbal drugs | |
dc.subject | intravital imaging | |
dc.title | Nanocurcumin-Loaded UCNPs for Cancer Theranostics: Physicochemical Properties, In Vitro Toxicity, and In Vivo Imaging Studies | |
dc.type | Article |