Biglycan and chondroitin sulfate play pivotal roles in bone toughness via retaining bound water in bone mineral matrix

Date
2020-09-28
Authors
Hua, Rui
Ni, Qingwen
Eliason, Travis D.
Han, Yan
Gu, Sumin
Nicolella, Daniel P.
Wang, Xiaodu
Jiang, Jean X.
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract

Recent in vitro evidence shows that glycosaminoglycans (GAGs) and proteoglycans (PGs) in bone matrix may functionally be involved in the tissue-level toughness of bone. In this study, we showed the effect of biglycan (Bgn), a small leucine-rich proteoglycan enriched in extracellular matrix of bone and the associated GAG subtype, chondroitin sulfate (CS), on the toughness of bone in vivo, using wild-type (WT) and Bgn deficient mice. The amount of total GAGs and CS in the mineralized compartment of Bgn KO mouse bone matrix decreased significantly, associated with the reduction of the toughness of bone, in comparison with those of WT mice. However, such differences between WT and Bgn KO mice diminished once the bound water was removed from bone matrix. In addition, CS was identified as the major subtype in bone matrix. We then supplemented CS to both WT and Bgn KO mice to test whether supplemental GAGs could improve the tissue-level toughness of bone. After intradermal administration of CS, the toughness of WT bone was greatly improved, with the GAGs and bound water amount in the bone matrix increased, while such improvement was not observed in Bgn KO mice or with supplementation of dermatan sulfate (DS). Moreover, CS supplemented WT mice exhibited higher bone mineral density and reduced osteoclastogenesis. Interestingly, Bgn KO bone did not show such differences irrespective of the intradermal administration of CS. In summary, the results of this study suggest that Bgn and CS in bone matrix play a pivotal role in imparting the toughness to bone most likely via retaining bound water in bone matrix. Moreover, supplementation of CS improves the toughness of bone in mouse models.

Description
Article available from publisher at https://www.sciencedirect.com/science/article/pii/S0945053X20300895?via%3Dihub.
Keywords
Biglycan, Chondroitin sulfate, Bone matrix, Bone toughness, Bound water
Citation
Hua, R., Ni, Q., Eliason, T. D., Han, Y., Gu, S., Nicolella, D. P., ... & Jiang, J. X. (2020). Biglycan and chondroitin sulfate play pivotal roles in bone toughness via retaining bound water in bone mineral matrix. Matrix Biology, 94, 95-109.
Department
Mechanical Engineering