A Customized Monkeypox Virus Genomic Database (MPXV DB v1.0) for Rapid Sequence Analysis and Phylogenomic Discoveries in CLC Microbial Genomics

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dc.contributor.authorShen-Gunther, Jane
dc.contributor.authorCai, Hong
dc.contributor.authorWang, Yufeng
dc.date.accessioned2023-01-20T14:23:27Z
dc.date.available2023-01-20T14:23:27Z
dc.date.issued2022-12-22
dc.date.updated2023-01-20T14:23:28Z
dc.description.abstractMonkeypox has been a neglected, zoonotic tropical disease for over 50 years. Since the 2022 global outbreak, hundreds of human clinical samples have been subjected to next-generation sequencing (NGS) worldwide with raw data deposited in public repositories. However, sequence analysis for in-depth investigation of viral evolution remains hindered by the lack of a curated, whole genome Monkeypox virus (MPXV) database (DB) and efficient bioinformatics pipelines. To address this, we developed a customized MPXV DB for integration with "ready-to-use" workflows in the CLC Microbial Genomics Module for whole genomic and metagenomic analysis. After database construction (218 MPXV genomes), whole genome alignment, pairwise comparison, and evolutionary analysis of all genomes were analyzed to autogenerate tabular outputs and visual displays (collective runtime: 16 min). The clinical utility of the MPXV DB was demonstrated by using a Chimpanzee fecal, hybrid-capture NGS dataset (publicly available) for metagenomic, phylogenomic, and viral/host integration analysis. The clinically relevant MPXV DB embedded in CLC workflows proved to be a rapid method of sequence analysis useful for phylogenomic exploration and a wide range of applications in translational science.
dc.description.departmentMolecular Microbiology and Immunology
dc.identifierdoi: 10.3390/v15010040
dc.identifier.citationViruses 15 (1): 40 (2023)
dc.identifier.urihttps://hdl.handle.net/20.500.12588/1590
dc.rightsAttribution 4.0 United States
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectbioinformatics
dc.subjectdisease outbreaks
dc.subjectmonkeypox
dc.subjectmonkeypox virus
dc.subjectnext generation sequencing
dc.subjectphylogeny
dc.subjectpoxvirus
dc.subjecttaxonomic classification
dc.subjectvirus database
dc.titleA Customized Monkeypox Virus Genomic Database (MPXV DB v1.0) for Rapid Sequence Analysis and Phylogenomic Discoveries in CLC Microbial Genomics
dc.typeArticle

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