Animal Model Dependent Response to Pentagalloyl Glucose in Murine Abdominal Aortic Injury

Date
2021-01-09
Authors
Anderson, Jennifer L.
Niedert, Elizabeth E.
Patnaik, Sourav S.
Tang, Renxiang
Holloway, Riley L.
Osteguin, Vangelina
Finol, Ender A.
Goergen, Craig J.
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Abstract

Abdominal aortic aneurysms (AAAs) are a local dilation of the aorta and are associated with significant mortality due to rupture and treatment complications. There is a need for less invasive treatments to prevent aneurysm growth and rupture. In this study, we used two experimental murine models to evaluate the potential of pentagalloyl glucose (PGG), which is a polyphenolic tannin that binds to and crosslinks elastin and collagen, to preserve aortic compliance. Animals underwent surgical aortic injury and received 0.3% PGG or saline treatment on the adventitial surface of the infrarenal aorta. Seventeen mice underwent topical elastase injury, and 14 mice underwent topical calcium chloride injury. We collected high-frequency ultrasound images before surgery and at 3–4 timepoints after. There was no difference in the in vivo effective maximum diameter due to PGG treatment for either model. However, the CaCl2 model had significantly higher Green–Lagrange circumferential cyclic strain in PGG-treated animals (p < 0.05). While ex vivo pressure-inflation testing showed no difference between groups in either model, histology revealed reduced calcium deposits in the PGG treatment group with the CaCl2 model. These findings highlight the continued need for improved understanding of PGG's effects on the extracellular matrix and suggest that PGG may reduce arterial calcium accumulation.

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Keywords
abdominal aortic aneurysms, pentagalloyl glucose, ultrasound, elastin
Citation
Journal of Clinical Medicine 10 (2): 219 (2021)
Department
Mechanical Engineering