Knockdown Indian Hedgehog (Ihh) does not delay Fibular Fracture Healing in genetic deleted Ihh mice and pharmaceutical inhibited Ihh Mice

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dc.contributor.authorLi, Shengchun
dc.contributor.authorXiang, Chuan
dc.contributor.authorWei, Xiaochun
dc.contributor.authorLi, Hongbin
dc.contributor.authorLi, Kai
dc.contributor.authorSun, Xiaojuan
dc.contributor.authorWang, Shaowei
dc.contributor.authorZhang, Min
dc.contributor.authorDeng, Jin
dc.contributor.authorWang, Xiaodu
dc.contributor.authorLi, Pengcui
dc.contributor.authorLi, Ruifang
dc.contributor.authorZhang, Yanxiang
dc.contributor.authorWei, Lei
dc.date.accessioned2021-01-28T21:12:01Z
dc.date.available2021-01-28T21:12:01Z
dc.date.issued2018-07-09
dc.descriptionArticle available from publisher at https://doi.org/10.1038/s41598-018-28657-7.en_US
dc.description.abstractThe objective of this study was to determine if Ihh is required for fracture healing. Fibular fracture was created in adult Col2a1-CreERT2; Ihhfl/fl mice. Ihhfl/fl mice received Tamoxifen (TM) to delete Ihh. WT mice received Cyclopamine to inhibit Hh pathway. Callus tissue properties and Ihh pathway were analyzed at 1, 2, and 3 weeks post-fracture by X-ray, micro-CT, mechanical test, RT-PCR and immunohistochemistry. Deleted Ihh was evidenced by the occurrence of growth plate closure in the Ihhfl/fl mice by X-ray 3 weeks after TM treatment. All mice showed fracture healing at 3 weeks post-operation. Histology analysis indicated that, compared to the control, cartilage area was less in fracture sites from Ihh deficient animals by either genetic deletion or drug inhibition at 1 and 2 weeks post-fracture. Ihh immunostaining and its mRNA level were diminished in the fracture callus in Ihh reduced mice. There was no significant difference in BV/TV, BMD and mechanical test. Interruption to Ihh pathway by either genetic or pharmaceutical approach didn’t affect fibular fracture healing in these mice. This surprised finding implicates that the deleted Ihh does not affect fracture healing in this model.en_US
dc.description.departmentMechanical Engineeringen_US
dc.description.sponsorshipThe project was supported by Grant R01AR059142 from NIH/NIAMS, NSFC 81572098, 81772415, 81772867, and 81601949, SXNSF 20150313012-6 and 201605D211024.en_US
dc.identifier.citationLi, S., Xiang, C., Wei, X. et al. Knockdown Indian Hedgehog (Ihh) does not delay Fibular Fracture Healing in genetic deleted Ihh mice and pharmaceutical inhibited Ihh Mice. Sci Rep 8, 10351 (2018). https://doi.org/10.1038/s41598-018-28657-7en_US
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/20.500.12588/223
dc.language.isoen_USen_US
dc.publisherNatureen_US
dc.relation.ispartofseriesScientific Reports;8
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.titleKnockdown Indian Hedgehog (Ihh) does not delay Fibular Fracture Healing in genetic deleted Ihh mice and pharmaceutical inhibited Ihh Miceen_US
dc.typeArticleen_US

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