Characterization of Local and Systemic Impact of Whitefly (Bemisia tabaci) Feeding and Whitefly-Transmitted Tomato Mottle Virus Infection on Tomato Leaves by Comprehensive Proteomics

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dc.contributor.authorOgden, Aaron J.
dc.contributor.authorBoukari, Wardatou
dc.contributor.authorNava, Alba
dc.contributor.authorLucinda, Natalia
dc.contributor.authorSunter, Garry
dc.contributor.authorCurtis, Wayne R.
dc.contributor.authorAdkins, Joshua N.
dc.contributor.authorPolston, Jane E.
dc.date.accessioned2021-04-19T15:23:29Z
dc.date.available2021-04-19T15:23:29Z
dc.date.issued2020-09-30
dc.date.updated2021-04-19T15:23:29Z
dc.description.abstractTomato mottle virus (ToMoV) is a single-stranded DNA (ssDNA) begomovirus transmitted to solanaceous crops by the whitefly species complex (Bemisia tabaci), causing stunted growth, leaf mottling, and reduced yield. Using a genetic repertoire of seven genes, ToMoV pathogenesis includes the manipulation of multiple plant biological processes to circumvent antiviral defenses. To further understand the effects of whitefly feeding and whitefly-transmitted ToMoV infection on tomato plants (Solanum lycopersicum 'Florida Lanai'), we generated comprehensive protein profiles of leaves subjected to feeding by either viruliferous whiteflies harboring ToMoV, or non-viruliferous whiteflies, or a no-feeding control. The effects of whitefly feeding and ToMoV infection were measured both locally and systemically by sampling either a mature leaf directly from the site of clip-cage confined whitefly feeding, or from a newly formed leaf 10 days post feeding (dpf). At 3 dpf, tomato's response to ToMoV included proteins associated with translation initiation and elongation as well as plasmodesmata dynamics. In contrast, systemic impacts of ToMoV on younger leaves 10 dpf were more pronounced and included a virus-specific change in plant proteins associated with mRNA maturation and export, RNA-dependent DNA methylation, and other antiviral plant processes. Our analysis supports previous findings and provides novel insight into tomato's local and systemic response to whitefly feeding and ToMoV infection.
dc.description.departmentBiology
dc.identifierdoi: 10.3390/ijms21197241
dc.identifier.citationInternational Journal of Molecular Sciences 21 (19): 7241 (2020)
dc.identifier.urihttps://hdl.handle.net/20.500.12588/523
dc.rightsAttribution 4.0 United States
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjecttomato
dc.subjecttomato mottle virus
dc.subjectRNA directed DNA methylation
dc.subjecttranscriptional gene silencing
dc.subjectBegomovirus
dc.subjectGeminivirus
dc.subjectBemisia tabaci
dc.subjectwhitefly
dc.subjecttomato
dc.subjectpathogenesis
dc.subjectproteomics
dc.titleCharacterization of Local and Systemic Impact of Whitefly (Bemisia tabaci) Feeding and Whitefly-Transmitted Tomato Mottle Virus Infection on Tomato Leaves by Comprehensive Proteomics
dc.typeArticle

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