Ocular trauma - induced changes in aqueous and plasma biomarker expression levels

Hernandez, Jessica Marie

Ocular trauma - induced changes in aqueous and plasma biomarker expression levels

dc.contributor.advisor	Reilly, Matthew A.
dc.contributor.advisor	Glickman, Randolph D.
dc.contributor.author	Hernandez, Jessica Marie
dc.contributor.committeeMember	Gray, Walter
dc.contributor.committeeMember	Tang, Liang
dc.date.accessioned	2024-02-09T22:24:56Z
dc.date.available	2024-02-09T22:24:56Z
dc.date.issued	2015
dc.description	This item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstract	The effect of varied blast overpressure on the visual system pathology was studied in 22 male Dutch Belted rabbits weighing 1.5 – 2.5 kg. Blast overpressure (BOP) of 8, 12, and 17 psi (55, 83, and 117 kPa, respectively) was simulated using a compartmentalized, pressure driven shock tube, aiming to induce sub-lethal ocular trauma. Trauma-related protein biomarkers were found using Milliplex Rat Cytokine/Chemokine (RECYMAG65K27PMX) and Human Neurological Disorders Panels (HND1MAG-39K) in aqueous humor (100 ?l) and blood plasma (2 ml) samples, obtained from anesthetized animals following primary blast ocular trauma. Parameters used were baseline, 24, and 48 hours, along with baseline, 3, 24, and 48 hours, for aqueous humor and blood plasma, respectively. Biomarkers concentrations were normalized for all animals subjected to BOP for each time point (n=22) (Fig. 4). Fold changes were plotted as a function of blast impulse, portraying significant linear regressions using a Tobit model from samples in R software 3.1.3. Linear fits represent biomarker expression levels for the proteins NGF, NSE, G-CSF, IL-4, IL-13, IL-12p70, MCP-1, LIX, and MIP-2 at each sampling time. Biomarker concentrations previously mentioned, were plotted against retinal/corneal thickness and intraocular pressure (IOP) measurements, of alike animals. Significant biomarkers which appeared to correlate with sub-lethal ocular trauma, seen in retinal thickness (RT), corneal thickness (CT), and IOP changes, were NGF, NSE, IL-4, IL-13, IL-12p70, and LIX. These findings suggest that biomarker identification may aid in the development of diagnostic tools for assessing blast injury to prevent or mitigate serious sequelae such as blindness, retinal hemorrhages, impairment of ocular movement and reflexes, and possible retinal detachment.
dc.description.department	Biomedical Engineering
dc.format.extent	56 pages
dc.format.mimetype	application/pdf
dc.identifier.isbn	9781339034324
dc.identifier.uri	https://hdl.handle.net/20.500.12588/3968
dc.language	en
dc.subject	Biomarkers
dc.subject	Immunoassays
dc.subject	Ocular Trauma
dc.subject	Primary Blast
dc.subject	Risk Curves
dc.subject	Traumatic Brain Injury
dc.subject	Blast overpressure
dc.subject	NGF
dc.subject	NSE
dc.subject	G-CSF
dc.subject	IL-4
dc.subject	IL-13
dc.subject	IL-12p70
dc.subject	MCP-1
dc.subject	LIX
dc.subject	MIP-2
dc.subject.classification	Biomedical engineering
dc.subject.classification	Biology
dc.subject.classification	Biostatistics
dc.subject.lcsh	Eye -- Wounds and injuries -- Treatment
dc.subject.lcsh	Biochemical markers
dc.subject.lcsh	Immunoassay
dc.title	Ocular trauma - induced changes in aqueous and plasma biomarker expression levels
dc.type	Thesis
dc.type.dcmi	Text
dcterms.accessRights	pq_closed
thesis.degree.department	Biomedical Engineering
thesis.degree.grantor	University of Texas at San Antonio
thesis.degree.level	Masters
thesis.degree.name	Master of Science