Modeling Dual Species Candida Albicans / Streptococcus Gordonii Oral Biofilms and Their Associated Resistance to Antimicrobial Treatment

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dc.contributor.advisorLopez-Ribot, Jose L.
dc.contributor.authorMontelongo-Jauregui, Daniel
dc.contributor.committeeMemberKlose, Karl E.
dc.contributor.committeeMemberSaville, Stephen P.
dc.contributor.committeeMemberWormley, Jr., Floyd L.
dc.contributor.committeeMemberPierce, Christopher G.
dc.creator.orcidhttps://orcid.org/0000-0001-6079-4277
dc.date.accessioned2024-02-12T18:27:52Z
dc.date.available2019-12-13
dc.date.available2024-02-12T18:27:52Z
dc.date.issued2018
dc.descriptionThis item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstractCandida albicans is a commensal opportunistic fungal pathogen that resides in mucosal surfaces of the human host, including the oral cavity. As a commensal, C. albicans has been known to interact with the microbiota already found in oral niches and such interactions could potentially affect its colonization and pathogenicity. Streptococcus gordonii, a bacterial organism described as an early colonizer of oral microbiota, has been noted to intimately interact with C. albicans by directly binding to hyphal adhesins. Moreover, S. gordonii has been described to induce filamentation in C. albicans, a key fungal virulence factor. In this work, we describe the development of two in vitro models that permit the study of such fungal-bacterial interactions during the formation of dual-species biofilms. The first one is an adaptation of a 96-well microtiter plate model previously developed for the formation of C. albicans biofilms; whereas the second one involves the growth of mixed biofilms on titanium alloy, the most commonly used biomaterial in dental implants. These models were developed with the use of BMM (Basal Medium Mucin) synthetic saliva in order to simulate physiological conditions found within the oral cavity. Using these in vitro models, we further characterized these fungal-bacterial interactions at the molecular level using C. albicans deletion mutant strains.
dc.description.departmentIntegrative Biology
dc.format.extent134 pages
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12588/4643
dc.languageen
dc.subjectAntimicrobial resistance
dc.subjectC. albicans
dc.subjectImplant material
dc.subjectMixed biofilms
dc.subjectOral biofilms
dc.subjectS. gordonii
dc.subject.classificationMicrobiology
dc.titleModeling Dual Species Candida Albicans / Streptococcus Gordonii Oral Biofilms and Their Associated Resistance to Antimicrobial Treatment
dc.typeThesis
dc.type.dcmiText
dcterms.accessRightspq_closed
thesis.degree.departmentIntegrative Biology
thesis.degree.grantorUniversity of Texas at San Antonio
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

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