Neutrophil Extracellular Traps in the Rhesus Macaque Model of Ebolavirus Disease

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dc.contributor.advisorHeidner, Hans
dc.contributor.authorStaples, Hilary Mace
dc.contributor.committeeMemberCardona, Astrid
dc.contributor.committeeMemberCarrion, Ricardo
dc.date.accessioned2024-03-08T15:42:54Z
dc.date.available2024-03-08T15:42:54Z
dc.date.issued2020
dc.descriptionThis item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstractNeutrophil extracellular traps (NETs) are one of several mechanisms neutrophils employ in response to microbial infection and are composed of decondensed chromatin studded with antimicrobial peptides and proteases that have been expelled to the extracellular space. In the 15 years since they were first identified for their antibacterial effects, NETs have also been implicated in the exacerbation of both infectious and non-infectious diseases through the development of coagulation disorders, tissue destruction, organ dysfunction, auto-antibodies, and the perpetuation of a hyper-inflammatory state. Several of these pathologies are consistent with those observed in Ebolavirus disease, e.g. coagulation disorders and organ dysfunction. In order to investigate whether NETs form in the context of Ebolavirus disease, multiple means of interrogation aimed at detecting markers of NETs in plasma and tissues derived from Ebola-infected Rhesus macaques were developed, including detection of MPO-DNA complexes and citrullinated histone H3 via sandwich ELISA as well as immunofluorescence assay for the detection of co-localized decondensed chromatin, citrullinated histone H3, and neutrophil elastase in tissue samples. Preliminary data indicate NETs do form in the context of Ebola infection beginning at 5 days post-infection in the Rhesus macaque, suggesting a potential role for NETs in Ebolavirus disease pathology.
dc.description.departmentIntegrative Biology
dc.format.extent93 pages
dc.format.mimetypeapplication/pdf
dc.identifier.isbn9798557049788
dc.identifier.urihttps://hdl.handle.net/20.500.12588/5613
dc.languageen
dc.subjectEbolavirus
dc.subjectEbolavirus disease
dc.subjectNETs
dc.subjectNeutrophils
dc.subjectRhesus macaque
dc.subject.classificationImmunology
dc.subject.classificationVirology
dc.titleNeutrophil Extracellular Traps in the Rhesus Macaque Model of Ebolavirus Disease
dc.typeThesis
dc.type.dcmiText
dcterms.accessRightspq_closed
thesis.degree.departmentIntegrative Biology
thesis.degree.grantorUniversity of Texas at San Antonio
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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