Clonal Expansion of Environmental Triazole Resistant Aspergillus fumigatus in Iran

Date

2020-10-01

Authors

Ahangarkani, Fatemeh
Badali, Hamid
Abbasi, Kiana
Nabili, Mojtaba
Khodavaisy, Sadegh
de Groot, Theun
Meis, Jacques F.

Journal Title

Journal ISSN

Volume Title

Publisher

MDPI

Abstract

Azole-resistance in Aspergillus fumigatus is a worldwide medical concern complicating the management of aspergillosis (IA). Herein, we report the clonal spread of environmental triazole resistant A. fumigatus isolates in Iran. In this study, 63 A. fumigatus isolates were collected from 300 compost samples plated on Sabouraud dextrose agar supplemented with itraconazole (ITR) and voriconazole (VOR). Forty-four isolates had the TR34/L98H mutation and three isolates a TR46/Y121F/T289A resistance mechanism, while two isolates harbored a M172V substitution in cyp51A. Fourteen azole resistant isolates had no mutations in cyp51A. We found that 41 out of 44 A. fumigatus strains with the TR34/L98H mutation, isolated from compost in 13 different Iranian cities, shared the same allele across all nine examined microsatellite loci. Clonal expansion of triazole resistant A. fumigatus in this study emphasizes the importance of establishing antifungal resistance surveillance studies to monitor clinical Aspergillus isolates in Iran, as well as screening for azole resistance in environmental A. fumigatus isolates.

Description

Keywords

Aspergillus fumigatus, azole resistance, compost, TR34/L98H, TR46/Y121F/T289A, Aspergillus fumigatus, azole resistance, compost, TR34/L98H, TR46/Y121F/T289A

Citation

Ahangarkani, F., Badali, H., Abbasi, K., Nabili, M., Khodavaisy, S., de Groot, T., & Meis, J. F. (2020). Clonal Expansion of Environmental Triazole Resistant Aspergillus fumigatus in Iran. Journal of Fungi, 6(4), 199. MDPI AG. Retrieved from http://dx.doi.org/10.3390/jof6040199

Department

Molecular Microbiology and Immunology