Transcription Regulation of Candida albicans Examining Filamentation Following Hyphae Induction

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dc.contributor.advisorSaville, Stephen
dc.contributor.authorKeith, Dwayne
dc.contributor.committeeMemberLopez-Ribot, Jose
dc.contributor.committeeMemberRamos, William
dc.date.accessioned2024-02-12T14:42:12Z
dc.date.available2024-02-12T14:42:12Z
dc.date.issued2020
dc.descriptionThis item is available only to currently enrolled UTSA students, faculty or staff. To download, navigate to Log In in the top right-hand corner of this screen, then select Log in with my UTSA ID.
dc.description.abstractCandida albicans is a fungal pathogen that is part of the human microbiota. It is an opportunistic organism that grows on mucosal surfaces of the gastrointestinal and genitourinary tracts. A few of the common diseases caused by C. albicans are thrush, yeast infections, multiple types of candidiasis, and is also known to cause life threatening infections in the immunocompromised. Hyphae morphogenesis, changing from yeast to hyphae, is key to its virulence. The ability to form hyphae allows C. albicans to form biofilms, evade phagocytosis by the immune system, and penetrate tissue eventually leading to systemic infections. UME6 is a transcriptional factor that plays a direct role in hyphal elongation and germ tube formation. In a previous study, it was found that BRG1 could not filament on YPD when inserted into a ume6Δ mutant, these results were used to determined that UME6 was downstream of BRG1. We decided to investigate this relationship further and inserted the UME6 gene into a plasmid that had a tet-operator and trans-activator to regulate expression, then introduced the tet-UME6 allele into a brg1Δ strain and the previously engineered tet-BRG1 allele into a ume6Δ strain. We then subjected the brgΔ-tet-UME6 and ume6Δ-tet-BRG1 strains to multiple hyphae inducing conditions. We than determined that UME6 and BRG1 are both required by the other to filament except under one hyphae inducing condition. From these experiments we concluded that both genes form a feedback loop, although more research is required.
dc.description.departmentIntegrative Biology
dc.format.extent44 pages
dc.format.mimetypeapplication/pdf
dc.identifier.isbn9798557050142
dc.identifier.urihttps://hdl.handle.net/20.500.12588/4237
dc.languageen
dc.subjectTranscription regulation
dc.subjectCandida albicans
dc.subjectFilamentation
dc.subjectHyphae induction
dc.subject.classificationMicrobiology
dc.subject.classificationPathology
dc.subject.classificationMolecular biology
dc.subject.classificationParasitology
dc.titleTranscription Regulation of Candida albicans Examining Filamentation Following Hyphae Induction
dc.typeThesis
dc.type.dcmiText
dcterms.accessRightspq_closed
thesis.degree.departmentIntegrative Biology
thesis.degree.grantorUniversity of Texas at San Antonio
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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